Faculty


Name Meng-Shiou Lee (李孟修) Meng-Shiou Lee (李孟修)
Title Associate Professor
cONTACT Tel: +886-4-22053366 ext. 5208
E-mail: leemengshiou@mail.cmu.edu.tw
Education
  1. Ph.D. Graduate Institute Biotechnology, National Chung Hsing University, Taiwan. 1998-2004.
  2. M.S. Graduate Institute of Food Science and Technology, Tunghai University, Taiwan. 1994-1996.
  3. B.S. Department of Food Science, Tunghai University, Taiwan. 1990-1994.
RESEARCH INTERESTS
  1. Characterization of Chicken anemia virus (CAV) viral proteinVP1, VP2 for vaccine application, studying of structure biology of CAV, mechanism elucidating of VP3 on anticancer activities.
  2. Development of an efficient bioprocess for recombinant protein production using insect larvae, insect cell culture, E. coli, plant hairy roots.
  3. Development of molecular and immunological assay for diagnosis purpose
  4. Development of rapid, sensitive and specific molecular methods for authentication of medicinal herbs.
  5. Application of bio-Chip on clinical diagnosis of Chinese medicine.
LAMP for Chicken Anemia Virus detectionLAMP for Chicken Anemia Virus detection
(Journal of Applied Microbiology 2009; doi:10.1111/j.1365-2672.2009.04481.x
Overproduction of chicken anemia virus viral VP1, VP2 proteins in E. coli for developing diagnoctic kit. Overproduction of chicken anemia virus viral VP1, VP2 proteins in E. coli for developing diagnoctic kit.
(Process Biochemistry 2009; 44: 390–395)
Expression of CAV VP2-GFP in mammalian cell for investigation of subcellular localization.Expression of CAV VP2-GFP in mammalian cell for investigation of subcellular localization.
(ongoing project in 2009)
PROFESSIONAL CAREER
  1. Department of Chemistry, National Taiwan University, Taiwan. Associate Professor. 1976.
  2. Department of  Chemistry, National Taiwan University, Taiwan. Professor. 1980.
  3. Faculty of Engineering, Kyoto University, Japan. Visiting Professor. 1984.
  4. Department of Pharmacy, Kyoto University, Japan. Visiting Professor. 1993.
  5. Agricultural Biotechnology Research Center, Academia Sinica, Taiwan. Adjunct Appointment Research Fellow. 2005.
  6. School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taiwan. Chair Professor. 2007.
POSTDOCTORAL RESEARCH and PROFESSIONALCAREER

PUBLICATIONS:

  1. C.-H. Huang, G.-H. Lai, M.-S. Lee, Wen-Hsin Lin, Y.-Y. Lien, S.-C. Hsueh, J.-Y. Kao, Wen-Te Chang, T.-C. Lu, W.-N. Lin, H.-J. Chen, Meng-Shiou Lee*, Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of chicken anemia virus, JOURNAL OF APPLIED MICROBIOLOGY, 2010 Mar, 108():917-924
  2. Wen-Neng Lin, Hsiu-Ying Lu, Meng-Shiou Lee, Shih-Ying Yang, Hsi-Jien Chen, Yuan Shiun Chang、Wen-Te Chang*, Evaluation of the cultivation age of dried ginseng radix and its commercial products by using 1H-NMR fingerprint analysis, AMERICAN JOURNAL OF CHINESE MEDICINE, 2010 Jan, 38(1):205-218
  3. Meng-Shiou Lee, Yi-Chiu Lin, Guan-Hua Lai, Su-Yaun Lai, Min-Ying Wang*, One-step reverse transcription loopmediated isothermal amplification for detection of infectious bursal disease virus, CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETER, 2010 ,():
  4. Chi-Hung Huang , Guan-Hua Lai, Meng-Shiunn Lee, Wen-Hsin Lin, Yi-Yang Lien, Shu-Chin Hsueh, Jung-Yie Kao, Wen-Te Chang, Tsung-Chi Lu, Wen-Neng Lin, Hsi-Jien Chen, Meng-Shiou Lee*. (2009) Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of chicken anemia virus. Journal of Applied Microbiology doi: 10.1111/j.1365-2672.2009.04481.x (Accepted, in press, Correspondence author)
  5. Meng-Shiou Lee, Yi-Yang Lien, Shin-Huei Feng, Ray-Ling Huang, Ming-Cheng Tsai,Wen-Te Chang, Hsi-Jien Chen. (2009) Production of chicken anemia virus (CAV) VP1 and VP2 protein expressed by recombinant Escherichia coli. Process Biochemistry. 44:390-395. (SCI; II) (Impact factor:2.336)
  6. Wen-Neng Lin, Hsiu-Ying Lu, Meng-Shiou Lee, Shih-Ying Yang, Hsi-Jien Chen, Yuan-Shiun Chang, Wen-Te Chang. (2009) Evaluation of the cultivation age of dried ginseng radix and its commercial products by using 1H-NMR fingerprint analysis. American Journal of Chinese Medicine (Accepted, in press)
  7. Jung Chao, Tsung-Chun Lu, Jiunn-Wang Liao, Tai-Hung Huang, Meng-Shiou Lee, Hao-Yuan Cheng, Li-Kang Ho, Chao-Lin Kuo, Wen-Huang Peng. (2009) Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice. Journal of Ethnopharmacology doi:10.1016/j.jep.2009.06.024. (Accepted, in press)
  8. Meng-Shiou Lee, Shyue-Ru Doong, Su-Yuan Lai, Jin-Yi Ho and Min-Ying Wang. (2006) Processing of infectious bursal disease virus (IBDV) polyprotein and self-assembly of IBDV-like particles in Hi-5 cells. Biotechnol Prog. 22: 763-769. (SCI; JY) (Impact factor:2.224)
  9. Chien-Shiuh Chen, Shing-Yi Suen, Su-Yuan Lai, Gary Ro-Lin Chang, Tsung-Chi Lu, Meng-Shiou Lee, Min-Ying Wang. (2005). Purification of capsid-like particles of infectious bursal disease virus (IBDV)VP2 expressed in E. coli with a metal-ion affinity membrane system. Journal of Virological methods. 130: 51-8. (SCI; DB) (Impact factor:1.933)
  10. Meng-Shiou Lee, Min-Ying Wang and Su-yuan Lai.(2004). Characterization of particles formed by infectious bursal disease virus structural precursor protein, VPX, in Hi5 cells using immobizied metal-ion affinity chromatography an size-exclusion chromatography. Journal of Virological methods. 121:191-199. (SCI; DB) (Impact factor:1.933)
  11. Meng-Shiou Lee*, Su-Yuan Lai*, Hsia-Chin Chen, Ping-Chen Shen, Tzyy-Rong Jinn, Suey-Sheng Kao, Min-Ying Wang.(2004) Production and purification of immunogenic virus-like particles formed by the chimeric infectiou bursal disease virus structural protein, rVP2H, in insect larvae. Process Biochemistry. 39:571-577. ( *Contribution equally) (SCI; II) (Impact factor:2.336)
  12. Lee C. C, Ko T. P, Lee M. S, Chou C. C, Lai S. Y, Wang A. H, Wang M. Y. 2003. Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2. Acta Crystallogr D Biol Crystallogr. 59(Pt 7):1234-7. (SCI; FI) (Impact factor:1.687)
  13. Tu-Shen Cheng, Meng-Shiou Lee, Su-Yuan Lai, Shyue-Ru Doong, Min-Ying Wang. 2001. Separation of Pure Immunoreactive Virus-Like Particles Using Gel Filtration Chromatography Following Immobilized Metal Ion Affinity Chromatography. Biotechnol. Prog.17:318-325. (SCI; JY) (Impact factor:2.224)
  14. Wang, M.-Y., Kuo, Y.-Y., Lee, M.-S., Doong, S.-R., Ho, J.-Y. and Lee, L.-H. 2000. Self-Assembly of the Infectious Bursal Disease Virus Capsid Protein, rVP2, Expressed in Insect Cells and Purification of Immunogenic Chimeric rVP2H Particles by Immobilized Metal-Ion Affinity Chromatography ” Biotechnology and Bioengineering 67:104-111. (SCI; DB) (Impact factor:2.999)

CONFERENCE PUBLICATIONS:

  1. Reverse Transcription–Loop-Mediated Isothermal Amplification Assay for Easy Detection of Cymbidium Mosaic Virus, 15th Conference on Biochemical Engineering, Southern Taiwan University, 2010.6.25 ~ 2010.6.26. (台南,南台科技大學 E棟 13樓國際會議廳)
  2. Rapid identification of the medicinal plant Taxaracum formosanum and its adulterant by ribosomal DNA internal transcribed spacer (ITS)-primed PCR, 15th Conference on Biochemical Engineering, Southern Taiwan University, 2010.6.25 ~ 2010.6.26
  3. Rapid identification of the medicinal plant Cinnamomum osmophloeum and its adulterant by trnL gene using loop mediated isothermal amplification (LAMP), 15th Conference on Biochemical Engineering, Southern Taiwan University, 2010.6.25 ~ 2010.6.26
  4. Study of the Molecular Interactions between the Parasitized Plant and the Parasitic Plant Cuscuta japonica, Darwin 200: International Symposium on Global Biodiversity, Human Health and Well-Being, China Medical University, Taichung, Taiwan, 2009.12.3 ~ 2009.12.9
  5. Study the Immunomodulatory Activity of Semen Cuscutae and Identify the Active Components,Asia Pacific Biochemical Engineering Conference 2009, Kobe International Conference Center, Port Island, Kobe, Japan, 2009.11.24 ~ 2009.11.28
  6. Production of chicken anemia virus VP3 protein using recombinant Escherichia coli for development of cancer therapeutic agent, APBioChEC'09 (Asia Pacific Biochemical Engineering Conference 2009), Kobe International Conference Center, Port Island, Kobe, Japan, 2009.11.24 ~ 2009.11.28
  7. Discrimination between dried ginseng rootlet and root from its commercial products using a metabolomic strategy, Asia Pacific Biochemical Engineering Conference 2009, Kobe International Conference Center, Port Island, Kobe, Japan, 2009.11.24 ~ 2009.11.28
  8. Guan-Hua Lai, Chi-Hung Huang, Yi-Yang Lien, Shu-Chin Hsueh, His-Jien Chen, Jung-Yie Kao, Wen-Te Chang, Tsung-Chi Lu, Meng-Shiou Lee*. Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of chicken anemia virus. The 2 nd China Medical University & Asia University Joint Conference on Biotechnology. Taichung, Taiwan. March 16, 2009.
  9. Meng-Shiou Lee*, Yi-Yang Lien, Shin-Huei Feng, Wen-Te Chang, Ray-Ling Huang, His-Jien Chen. Expression and characterization of chicken anemia virus VP2 protein fused with green fluorescent protein in insect cells. The 14th Symposium of Young Asian Biochemical Engineers’Community (YABEC). Shibaura Institute of Technology, Toyosu Campus (Tokyo), Japan. Nov. 19- Dec. 1, 2008.
  10. How-Ping Chang, Wen-Chien Lu, Yi-Hsuan Wu, Meng-Shiou Lee, Wen-Te Chang*. Efficient production of catapol in Rehmannia glutinosa hairy root culture with adsorbents. The 14th Symposium of Young Asian Biochemical Engineers’Community (YABEC). Shibaura Institute of Technology, Toyosu Campus (Tokyo), Japan. Nov. 19- Dec. 1, 2008.
  11. Wen-Chih Liu, Hong-Zin Lee, Meng-Shiou Lee, Wen-Te Chang. Mechanism study of Citrus reticulate extract on the migration inhibition of human lung non-small carcinoma cell. The 23rd Symposium on Natural Products. China Medical University, Taichung, September 21, 2008.(台中,中國醫藥大學)
  12. Yi-Hsuan Wu, Wen-Te Chang, Min-Ying Wang, Meng-Shiou Lee*. Expressing chicken infectious bursal disease virus VP2 protein using transgenic plant hairy roots for the development of edible vaccine. The 23rd Symposium on Natural Products. China Medical University, Taichung, September 21, 2008.(台中,中國醫藥大學)
  13. Pei-Shan Li, Ting-Hsaing Chang, Meng-Shiou Lee, Fu-Shin Chueh, Chao-Lin Kou. The Preliminary Study on Plant Regeneration and Callus Induction in Hypericum japonicum. The 23rd Symposium on Natural Products. China Medical University, Taichung, September 21, 2008.(台中,中國醫藥大學)
  14. Meng-Shiou Lee*, Shin-Huei Feng, Yi-Yang Lien, Hsi-Jien Chen, Ray-Ling Huang. Expression and optimal production of N-terminus deletion mutants of VP1 and VP2 of chicken anemia virus (CAV) in Escherichia coli. 236th ACS National Meeting. Philadelphia, PA, August 17-21, 2008.
  15. Wen-Chien Lu, Yi-Hsuan Wu, Meng-Shiou Lee, Wen-Te Chang. Effect of Various Environmental Factors on Catalpol Production Using Hairy Root Culture of Rehmannia glutinosa L. 13th Biochemical Engineering conference. Yuan Ze University, Chung-Li, Taoyuan, June 27-28, 2008.(桃園、中壢,元智大學)
  16. Shin-Huei Feng, Yu-Chi Chen, Yi-Yang Lien, Hsi-Jien Chen, Ray-Ling Huang, Meng-Shiou Lee*. Construction of Recombinant Baculovirus and Expression of VP1 and VP2 Proteins of Chicken Anemia Virus in Insect Cell. 13th Biochemical Engineering conference. Yuan Ze University, Chung-Li, Taoyuan, June 27-28, 2008.(桃園、中壢,元智大學)
  17. Meng-Shiou Lee, Ray-Ling Huang, Hsi-Jien Chen. Process Investigation for Extraction of Bioactive Compounds in Hypericum perforatum (St. John’s Wort),Leiden University, The Netherlands on April 24-25, 2008.
  18. Wen-Hung Chen, Ray-Ling Huang, Hsi-Jien Chen,Meng-Shiou Lee*. A Comparison of the Effect of Polyethylene Glycol (PEG) and γ-Polyglutamic Acid (γ-PGA) on Precipitation of Virus Particles,第一屆中國醫藥大學生物科技研討會,中國醫藥大學B1國際會議廳,2008. 4. 7 11. Shyue-Ru Doong, Dau-Shiang Yang, Cheng-Chung Lee, Meng-Shiou Lee, Min-Ying Wang. The intrinsic nickel binding affinity of infectious bursal disease virus and untagged subviral particles. The 11th Biochemical Engineering Conference, Taiwan Symposium on Nanomedicine & Tissue Engineering, June 23-24, 2006. National Tsing Hua University. Taiwan
  19. J. Y. Ho, M. S. Lee, S. R. Doong, M. Y. Wang, S. Y. Lai. Processing of infectious bursal disease virus (IBDV) pVP2 promotes self-assembly of IBDV-like particles in Hi-5 cells. 19th Symposium of the protein society, Boston Marriott-Boston, MA, July 30-August 3, 2005.
  20. S. R. Doong, M. S. Lee, Y. C. Lin, H. K. Tsao, M, Y. Wang. Multi-site binding between capsid-like particles formed by a recombinant protein IBDV VP2 and immobilized Ni(II) ions. 19th Symposium of the protein society, Boston Marriott-Boston, MA, July 30-August 3, 2005.
  21. Meng-Shiou Lee, Shyue-Ru Doong, Su-yuan Lai and Min-Ying Wang. Characterization of particles formed by infectious bursal disease virus structural precursor protein, VPX, in Hi5 cells using immobizied metal-ion affinity chromatography an size-exclusion chromatography. American society for virology 22nd annual meeting, 2003, July 12-16, UC Davis, USA.
  22. Meng-Shiou Lee, Tu-Shen , Cheng, Su-Yuan Lai, Ming-ying Wang. Formation of virus-like particles by coexpressing two structure protein (VP2 and VP3) of infectious bursal disease virus in insect cells.ACS National Meeting, 2000, March 26-30.San Francisco, USA.
  23. J. Y. Ho, Y. C. Lin, Y. J. Dai, M. S. Lee, S. R. Doong, S. Y. Lai and M. Y. Wang. The effect of C-terminal and N-terminal ends of infectious bursal disease virus structurel protein, VP2, on its self-assembly in insect cells. American society for virology 22nd annual meeting, 2003, July 12-16, UC Davis, USA.
  24. Meng-Shiou Lee, Shyue-Ru Doong, Ji-Yi Ho, Su-Yuan Lai and Min-Ying Wang. Chromatographic analysis of the particles assembly of infectious bursal disease virus structural precursor protein VPX and its truncated mutants. The 8th symposium on Recent advances in Biophysics, 2002, May 22-25, Academia Sinica, Taipei.
  25. Chian New Chua, Meng-Shiou Lee, Su-Yuan Lai and Min-Ying Wang. Self-assembly of infectious bursal disease virus capsid protein, erVP2, expressed in E. coli and purification of chimeric erVP2H particles by immobizied metal-ion affinity chromatography. The 8th symposium on Recent advances in Biophysics, 2002, May 22-25, Academia Sinica, Taipei.
  26. Cheng-Chung Lee, Meng-Shiou Lee, Tzu-Ping Ko, Chia-Cheng Chou,Su-Yuan Lai, Min-Ying Wang and Andrew H.-J. Wang. Purification, crystallization, and preliminary x-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2. Tenth symposium on recent advances in cellular and molecular biology, 2002, January 25-28, peng kung, Taiwan.
  27. Yi-Hsin Cheng, Min-Ying Wang, Meng-Shiou Lee and Su-Yuan Lai, 2002. Expression, purification and characterization of infectious bursal disease virus (IBDV) structural protein VP3 in E. coli. The Seventeenth Joint Annual Conference of Biomedical Sciences (生物醫學聯合學術年會). March 23-24, Taipei, Taiwan.
  28. 王敏盈、李孟修、董學儒、曹恆光。The Conformational Effects of Infectious Bursal Disease Virus Recombinant Structural Protein rVP2 on Immobilized Metal-ion Affinity Chromatography Purification. Proceedings of the Fifth Conference on Biochemical Engineering, 2002, June 27-28, 桃園, 台灣。

RESEARCH GRANTS:

  1. Study of assembly mechanism of chicken infectious anemia viral capsid using assembly model ofrecombinant virus-like particles (I) [NSC95-2313-B-039-004-;$NTD 1,000,000; PI]
  2. Study of assembly mechanism of chicken infectious anemia viral capsid using assembly model ofrecombinant virus-like particles (II)[ NSC96-2313-B-276-001-MY3; $NTD 4,452,000; PI]

 

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